EXTRACTION, PARTIAL PURIFICATION AND SOME FACTORS AFFECTING MILK CLOTTING ACTIVITY OF A MILK COAGULANT PREPARED FROM SUNFLOWER (Helianthus annus) SEEDS
نویسندگان
چکیده
منابع مشابه
Characterization of partially purified milk‐clotting enzyme from sunflower (Helianthus annuus) seeds
This study was aimed to extract milk-clotting enzyme from sunflower seeds and to determine its potentiality for manufacturing white soft cheese from cows and goats milk. The seeds were blended and extracted using two types of buffers and milk-clotting and proteolytic activities were evaluated. The enzyme was partially purified using ammonium sulfate fractionation techniques. Results indicated t...
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Background and purpose: Lactoferrin is found in mucus, milk, and colostrum secretions and has antimicrobial activities, improves iron absorption, and enhances immune responses. Lactoferrin has the ability to degrade starch. The aim of this study was to investigate the preservation of milk lactoferrin enzymatic activity after purification by ion exchange chromatography. Materials and methods: ...
متن کامل[Study on the quantitative and qualitative composition of moulds colonizing sunflower seeds (Helianthus annus L.)].
INTRODUCTION The aim of study was to estimate the qualitative and quantitative composition of moulds colonizing sunflower seeds on RBCA, YpSs, DG18 media at 25, 37, and 45°C. MATERIAL Twenty samples of sunflower seeds (not roasted and not salted) were used for the analysis. The highest number of moulds were xerophilic species isolated on DG18 at 25°C (33), followed by mesophilic species (29) ...
متن کاملPurification and Characterization of Milk Clotting Enzyme Produced by Rhizomucor Rmiehei
Milk clotting enzyme (M CE) produced by: Rhizomucor miehei was purified and characterized.The enzyme was purified 220.29-fold with specific activity about 14444.2 U/mg protein byultrafiltration, ammonium sulfate fractionation, Sephacryl S-300 chromatography. The maximumenzyme activity was at 65°C.The milk clotting activity was decreased steadily as pH is increased and indicated maximumactivity ...
متن کاملMilk-clotting Enzymes from Microorganisms.
A total of 230 cultures of fungi and 43 cultures of bacteria, isolated from such sources as soil, butter, and milk, were screened for their milk-clotting activity. The fungi were cultivated on semisolid media, and the bacteria were grown in milk media in shake culture. Phytic acid, added as calcium phytate, was found to stimulate production of the enzyme in most of the bacterial isolates. Prote...
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ژورنال
عنوان ژورنال: Zagazig Journal of Agricultural Research
سال: 2019
ISSN: 1110-0338
DOI: 10.21608/zjar.2019.33399